Workpackage 4: Standardization of DNA metabarcoding for copepod species

Objectives
  • Design PCR-primers for DNA metabarcoding of copepods
  • Sequence bulk samples of copepods using next generation sequencing
 
Activities
  • Task 4.1: In-silico primer design for DNA metabarcoding
  • Task 4.2: PCR and amplicon next-generation sequencing
 
Expected key results
  • Sequence of PCR primers for DNA metabarcoding of copepods
  • Optimized PCR conditions
  • Raw next generation sequencing data
 
Deliverables
  • D4.1: Sequence of PCR primers for DNA metabarcoding of copepods and optimized PCR conditions
  • (month 3)
  • D4.2: List of bulk samples and pseudo-samples sequenced (month 16).